Journal: British Journal of Nutrition

Article Title: Identification of epicatechin as one of the key bioactive constituents of polyphenol-enriched extracts that demonstrate an anti-allergic effect in a murine model of food allergy

doi: 10.1017/s0007114514000877

Figure Lengend Snippet: Fig. 1. Ability of two different polyphenol-enriched apple extracts to affect allergy symptoms in sensitised mice. (a) Ovalbumin (OVA)-induced murine model of food allergy and timing of dietary intervention. (b) Allergy symptom scores after intervention with polyphenol-enriched apple extracts. (c) Distribution of allergy symptom scores (scale 0–4) in the different groups (0, ; 1, ; 2, ; 3, ; 4, ), with 4 being the score indicating the severest symptom. (d) Drop in temperature (8C) recorded in the different groups before and after the challenge. (e) Concentration of OVA-specific IgG2a (mg/ml) in the different groups. Groups are rep- resented as negative control (Neg cont, ), positive control (Pos cont, ), apple extract A ( ) and apple extract B ( ). ** Mean value was significantly different from that of the positive control group (P,0·01).

Article Snippet: Serially diluted standard (monoclonal mouse anti-OVA IgG1 and monoclonal mouse anti-OVA IgG2a from Antibody Shop; Lucerna-Chem) and sera were incubated for 2 h at 378C, followed by incubation for 2 h with a HRP labelled goat antimouse IgG1 or IgG2a antibody (1:5000; Southern Biotech; Bioconcept).

Techniques: Concentration Assay, Negative Control, Positive Control